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A plasmid containing complete gene copied from human genome is introduced in a bacterial cell, but the bacteria is unable to make a working copy of the protein because

PRINCIPLES OF BIOTECHNOLOGY (Genetic & Bioprocess engineering)
NEET
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It cannot recognise the start codon in mRNA

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Of the absence of similar amino acids in bacteria

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Of the absence of similar splicing machinery

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The foreign gene cannot replicate in bacteria

Solution:

Eukaryotic genes contain introns (non-coding sequences) that are removed from the pre-mRNA through a process called splicing to produce functional mRNA. Prokaryotic cells, like bacteria, lack the necessary splicing machinery. Therefore, when a complete eukaryotic gene (with introns) is introduced into a bacterium, the bacterium transcribes the entire gene, including introns, into mRNA. Without splicing, this mRNA is translated into a non-functional or incorrect protein. To express eukaryotic proteins in bacteria, cDNA (complementary DNA), which is synthesized from spliced mRNA and thus lacks introns, is typically used.

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