Restriction site for PvuI in cloning vector, pBR322 is present in

Solution:

The cloning vector pBR322 is a widely used plasmid vector. It contains several restriction sites and two antibiotic resistance genes: ampicillin resistance (ampᴿ) and tetracycline resistance (tetᴿ). The restriction enzyme PvuI has a recognition site within the ampᴿ gene of pBR322. This allows for insertional inactivation of the ampicillin resistance gene if a foreign DNA fragment is ligated into this site, which is a common method for selecting recombinants.